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Siderophores are low molecular weight secondary metabolites produced by microorganisms under low iron stress as a specific iron chelator. In the present study, a rhizospheric bacterium was isolated from the rhizosphere of sesame plants from Salem district, Tamil Nadu, India and later identified as Bacillus subtilis LSBS2. It exhibited multiple plant-growth-promoting (PGP) traits such as hydrogen cyanide (HCN), ammonia, and indole acetic acid (IAA), and solubilized phosphate. The chrome azurol sulphonate (CAS) agar plate assay was used to screen the siderophore production of LSBS2 and quantitatively the isolate produced 296 mg/L of siderophores in succinic acid medium. Further characterization of the siderophore revealed that the isolate produced catecholate siderophore bacillibactin. A pot culture experiment was used to explore the effect of LSBS2 and its siderophore in promoting iron absorption and plant growth of Sesamum indicum L. Data from the present study revealed that the multifarious Bacillus sp. LSBS2 could be exploited as a potential bioinoculant for growth and yield improvement in S. indicum.