作者
Qi Zhang, Yu Lou, Jiaqi Yang, Junli Wang, Jie Feng, Yali Zhao, Lin Wang, Xing Huang, Qihan Fu, Mao Ye, Xiaozhen Zhang, Yiwen Chen, Ce Ma, Hongbin Ge, Jianing Wang, Jiangchao Wu, Tao Wei, Qi Chen, Junqing Wu, Chengxuan Yu, Yanyu Xiao, Xinhua Feng, Guoji Guo, Tingbo Liang, Xueli Bai
发表日期
2019/11/1
期刊
Gut
卷号
68
期号
11
出版商
BMJ Publishing Group
简介
Objective
Hepatocellular carcinoma (HCC) is heterogeneous, especially in multifocal tumours, which decreases the efficacy of clinical treatments. Understanding tumour heterogeneity is critical when developing novel treatment strategies. However, a comprehensive investigation of tumour heterogeneity in HCC is lacking, and the available evidence regarding tumour heterogeneity has not led to improvements in clinical practice.
Design
We harvested 42 samples from eight HCC patients and evaluated tumour heterogeneity using whole-exome sequencing, RNA sequencing, mass spectrometry-based proteomics and metabolomics, cytometry by time-of-flight, and single-cell analysis. Immunohistochemistry and quantitative polymerase chain reactions were performed to confirm the expression levels of genes. Three independent cohorts were further used to validate the findings.
Results
Tumour heterogeneity is …
学术搜索中的文章
Q Zhang, Y Lou, J Yang, J Wang, J Feng, Y Zhao… - Gut, 2019