作者
Kumar Gaurav, Sanu Arora, Paula Silva, Javier Sánchez-Martín, Richard Horsnell, Liangliang Gao, Gurcharn S Brar, Victoria Widrig, Jon Raupp, Narinder Singh, Shuangye Wu, Sandip M Kale, Catherine Chinoy, Paul Nicholson, Jesús Quiroz-Chávez, James Simmonds, Sadiye Hayta, Mark A Smedley, Wendy Harwood, Suzi Pearce, David Gilbert, Ngonidzashe Kangara, Catherine Gardener, Macarena Forner-Martínez, Jiaqian Liu, Guotai Yu, Scott Boden, Attilio Pascucci, Sreya Ghosh, Amber N Hafeez, Tom O’Hara, Joshua Waites, Jitender Cheema, Burkhard Steuernagel, Mehran Patpour, Annemarie Fejer Justesen, Shuyu Liu, Jackie C Rudd, Raz Avni, Amir Sharon, Barbara Steiner, Rizky Pasthika Kirana, Hermann Buerstmayr, Ali A Mehrabi, Firuza Y Nasyrova, Noam Chayut, Oadi Matny, Brian J Steffenson, Nitika Sandhu, Parveen Chhuneja, Evans Lagudah, Ahmed F Elkot, Simon Tyrrell, Xingdong Bian, Robert P Davey, Martin Simonsen, Leif Schauser, Vijay K Tiwari, H Randy Kutcher, Pierre Hucl, Aili Li, Deng-Cai Liu, Long Mao, Steven Xu, Gina Brown-Guedira, Justin Faris, Jan Dvorak, Ming-Cheng Luo, Ksenia Krasileva, Thomas Lux, Susanne Artmeier, Klaus FX Mayer, Cristobal Uauy, Martin Mascher, Alison R Bentley, Beat Keller, Jesse Poland, Brande BH Wulff
发表日期
2021/2/1
期刊
Biorxiv
页码范围
2021.01. 31.428788
出版商
Cold Spring Harbor Laboratory
简介
Aegilops tauschii, the diploid wild progenitor of the D-subgenome of bread wheat, constitutes a reservoir of genetic diversity for improving bread wheat performance and environmental resilience. To better define and understand this diversity, we sequenced 242 Ae. tauschii accessions and compared them to the wheat D-subgenome. We characterized a rare, geographically-restricted lineage of Ae. tauschii and discovered that it contributed to the wheat D-subgenome, thereby elucidating the origin of bread wheat from at least two independent hybridizations. We then used k-mer-based association mapping to identify discrete genomic regions with candidate genes for disease and pest resistance and demonstrated their functional transfer into wheat by transgenesis and wide crossing, including the generation of a library of ‘synthetic’ hexaploids incorporating diverse Ae. tauschii genomes. This pipeline permits rapid trait discovery in the diploid ancestor through to functional genetic validation in a hexaploid background amenable to breeding.
学术搜索中的文章
K Gaurav, S Arora, P Silva, J Sánchez-Martín… - Biorxiv, 2021