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Silver nanoparticles (Ag NPs) with anti-bacterial effects against the biofilm-producing bacteria Klebsiella pneumoniae (K. pneumoniae) were synthesized using the marine seaweed Gracilaria corticata (G. corticata). Physiochemical characterization using UV-spectrometer, fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) confirmed that the synthesized material consisted of Ag NPs. Morphological analysis using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) with energy-dispersive X-ray (EDX) analysis confirmed the shape, size, surface morphology, and available chemical composition of the Ag NPs. Furthermore, the rich phenolic and flavonoid content of G. corticata has excellent antioxidant activity, which was confirmed through gas chromatography-mass spectrometry (GC–MS) analysis. The highest antibacterial activity and biofilm reduction (88 %) of Ag …