作者
Andrew H Paterson, Curt L Brubaker, Jonathan F Wendel
发表日期
1993/6
期刊
Plant Molecular Biology Reporter
卷号
11
页码范围
122-127
出版商
Kluwer Academic Publishers
简介
Extraction of high-quality genomic DNA fromGossypium (cotton) species is difficult due to high levels of polysaccharide, oxidizable quinones, and other interfering substances. We describe a procedure that consistently permits isolation of cotton genomic DNA of satisfactory size and quality for RFLP and PCR analysis, as well as for most routine cloning applications. Several antioxidants, phenol-binding reagents, and phenol oxidase inhibitors are used throughout the procedure, and most polysaccharides are eliminated early in the procedure by isolation of nuclei.
引用总数
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AH Paterson, CL Brubaker, JF Wendel - Plant Molecular Biology Reporter, 1993