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The metabolic profile of the seeds of seven Chia (Salvia hispanica L.) populations, three commercial (two black, and one white) and four early flowering genotypes (G3, G8, G17, W13.1), was investigated by NMR spectroscopy. The research aimed at evaluating the chemical composition of the different genotypes both from a qualitative point of view, through the identification of the major classes of organic compounds by NMR analysis, and from a quantitative point of view, through the integration of NMR spectra followed by Principal Component Analysis and chemometrics. Results showed that apolar organic extracts were mainly composed of mono- and polyunsaturated fatty acids, such as α-linolenic acid, while polar organic extracts contained the sugars glucose, raffinose and sucrose as the main metabolites, together with caffeoyl derivatives, flavonoids, organic acids, and free amino acids. Tashinone I and 15,16 …