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Familial aggregation is a significant risk factor for the development of thyroid cancer and familial non‐medullary thyroid cancer (FNMTC) accounts for 5–7% of all NMTC. Whole exome sequencing analysis in the family affected by FNMTC with oncocytic features where our group previously identified a predisposing locus on chromosome 19p13.2, revealed a novel heterozygous mutation (c.400G > A, NM_012335; p.Gly134Ser) in exon 5 of MYO1F, mapping to the linkage locus. In the thyroid FRTL‐5 cell model stably expressing the mutant MYO1F p.Gly134Ser protein, we observed an altered mitochondrial network, with increased mitochondrial mass and a significant increase in both intracellular and extracellular reactive oxygen species, compared to cells expressing the wild‐type (wt) protein or carrying the empty vector. The mutation conferred a significant advantage in colony formation, invasion and anchorage …