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Quercus infectoria seeds were used in the present study, and fatty acid content in fixed oil was determined using GC-FID. The total amount of the oil present in the seed was 7.2%. The major fatty acid identified were oleic acid (58.13%), linoleic acid (19.84%), palmitic acid (15.99%), stearic acid (2.27%), α–linolenic acid (1.31%) and other constituents like heptadecanoic acid, cis-11-eicosenoic acid, cis-10-heptadecenoic acid, behenic, lignoceric and myristic acid with less the 1%. Due to its phenolic compounds and α-tocopherol, the oil exhibits its antioxidant activity. Antioxidant properties of the oil were determined via DPPH radical scavenging and β-carotene bleaching assay. The physical properties and UV and FT-IR spectra were also determined.