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The mpA gene, coding for the protein component of ribonuclease P (RNase P), was allocated to the dnaA region at 83 min of the E. coli K-12 map. This was accomplished through analysis of recombinant pBR322 plasmids, some of which complemented the temperature sensitivity of a strain carrying the rnpA 49 allele and restored the RNA processing activity. Although the temperature sensitivity of a strain carrying the rnp-241 allele could not be complemented by the rnpA⁺ plasmid, the RNA-processing activity was restored, suggesting that the rnp-241 mutation is allelic with rnpA49. In this analysis we also found two genes coding for proteins (60 and 50 kDal) of unknown function. The order of the genes located in this region is in the clockwise orientation: rpmH(5.4 kDal; ribosomal protein L34), rnpA (14 kDal; protein component of RNase P), agene for a 60-kDal protein (inner membrane protein), a gene for a 50-kDal …