作者
Luigi Biancone, Vincenzo Cantaluppi, Giuseppe Mauriello Romanazzi, Simona Russo, Federico Figliolini, Silvia Beltramo, Elisa Scalabrino, Maria Chiara Deregibus, Renato Romagnoli, Alessandro Franchello, Mauro Salizzoni, Paolo Cavallo Perin, Camillo Ricordi, Giuseppe Paolo Segoloni, Giovanni Camussi
发表日期
2006/2/27
期刊
Transplantation
卷号
81
期号
4
页码范围
511-518
出版商
LWW
简介
Background.
Recent data suggest that donor intraislet endothelial cells may survive islet transplantation and participate to the events that influence islet engraftment. However, the mechanisms that regulate islet endothelial behavior in this setting are poorly known.
Methods.
We obtained immortalized human (hIECs) and mouse (mIECs) islet endothelial cells by transfection with SV40-T-large antigen and studied the synthesis and response to Platelet-activating factor (PAF), a multipotent phospholipid that acts as endothelial mediator of both inflammation and angiogenesis.
Results.
HIECs showed typical endothelial markers such as expression of vWF, CD31, and CD105, uptake of acetylated-LDL and binding to ULE-A lectin. Moreover, they expressed nestin, the PAF-receptor and possess surface fenestrations and in vitro angiogenic ability of forming tubular structures on Matrigel. Likewise, mIECs showed expression of …
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L Biancone, V Cantaluppi, GM Romanazzi, S Russo… - Transplantation, 2006