作者
Rita De Gasperi, Chenglin Mo, Daniella Azulai, Zhiying Wang, Lauren M Harlow, Yating Du, Zachary Graham, Jiangping Pan, Xin‐hua Liu, Lei Guo, Bin Zhang, Fred Ko, Ashleigh M Raczkowski, William A Bauman, Chris N Goulbourne, Wei Zhao, Marco Brotto, Christopher P Cardozo
发表日期
2022/2
期刊
Journal of Cachexia, Sarcopenia and Muscle
卷号
13
期号
1
页码范围
454-466
简介
Background
The role of Numb, a protein that is important for cell fate and development and that, in human muscle, is expressed at reduced levels with advanced age, was investigated; adult mice skeletal muscle and its localization and function within myofibres were determined.
Methods
Numb expression was evaluated by western blot. Numb localization was determined by confocal microscopy. The effects of conditional knock out (cKO) of Numb and the closely related gene Numb‐like in skeletal muscle fibres were evaluated by in situ physiology, transmission and focused ion beam scanning electron microscopy, three‐dimensional reconstruction of mitochondria, lipidomics, and bulk RNA sequencing. Additional studies using primary mouse myotubes investigated the effects of Numb knockdown on cell fusion, mitochondrial function, and calcium transients.
Results
Numb protein expression was reduced by ~70 …
学术搜索中的文章
R De Gasperi, C Mo, D Azulai, Z Wang, LM Harlow… - Journal of Cachexia, Sarcopenia and Muscle, 2022