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N^ɛ‐Carboxymethyl‐lysine (CML) is a prominent advanced glycation end‐product which is not only found in vivo but also in food. It is known that a percentage of the dietary CML (dCML) is absorbed into the circulation and only partly excreted in the urine. Several studies have tried to measure how much dCML remains in tissues. However obstacles to interpreting the data have been found.

A new protocol which discriminates dCML from native CML (nCML) has been developed. Three CML isotopes with different mass‐to‐charge ratios were used: nCML N^ε‐carboxymethyl‐L‐lysine, dCML N^ε‐[¹³C]carboxy[¹³C]methyl‐L‐lysine and internal standard N^ε‐carboxymethyl‐L‐[4,4,5,5‐²H₄]lysine. Wild‐type (n = 7) and RAGE^−/− (n = 8) mice were fed for 30 days with either a control, or a BSA‐bound dCML‐enriched diet. Organs were analyzed for nCML and dCML using liquid chromatography …