作者
Jun Yang, Hui-Ping Bi, Wei-Juan Fan, Min Zhang, Hong-Xia Wang, Peng Zhang
发表日期
2011/12/1
期刊
Plant Science
卷号
181
期号
6
页码范围
701-711
出版商
Elsevier
简介
Efficient Agrobacterium tumefaciens-mediated transformation was developed using embryogenic suspension cell cultures of elite sweet potato (Ipomoea batatas [L.] Lam.) cultivars, including Ayamurasaki, Sushu2, Sushu9, Sushu11, Wanshu1, Xushu18 and Xushu22. Embryogenic suspension cultures were established in LCP medium using embryogenic calli induced from apical or axillary buds on an induction medium containing 2mgl−1 2,4-D. Suspension cultures were co-cultivated with A. tumefaciens strain LBA4404 harboring the binary plasmid pCAMBIA1301 with the hpt gene as a selectable marker and an intron-interrupted uidA gene as a visible marker. Several key steps of the sweet potato transformation system have been investigated and optimized, including the appropriate antibiotics and their concentrations for suppressing Agrobacterium growth and the optimal doses of hygromycin for transformant …
学术搜索中的文章
J Yang, HP Bi, WJ Fan, M Zhang, HX Wang, P Zhang - Plant Science, 2011