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Background and purpose:  The aims of this study were to investigate the anti‐cancer activity of SZ‐685C, an anthracycline analogue isolated from marine‐derived mangrove endophytic fungi, and to explore the molecular mechanisms underlying such activity.

Experimental approach:  The effect of SZ‐685C on the viability of cancer cell lines was investigated using the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) assay. SZ‐685C‐induced apoptosis was assessed by Annexin V‐fluorescein isothiocyanate/propidium iodide staining, terminal deoxynucleotidyl transferase‐mediated dUTP nick end labelling assay and analysis of caspase activation. The effect of SZ‐685C on the Akt/FOXO pathway was studied using Western blotting analysis, and the in vivo anti‐tumour efficacy was examined in an MDA‐MB‐435 breast cancer xenograft model.

Key results:  SZ‐685C suppressed the …