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Personality differences are widespread throughout the animal kingdom and can have important ecological and evolutionary consequences. Despite a rapidly increasing body of literature, large (marine) vertebrates remain underrepresented in personality research. Given their unique life history traits (e.g. slow growth rate, slow reproduction rate, long life span) and their pivotal role in ecosystem processes, this is an important gap in our current knowledge. Here we investigated consistency and plasticity in movement behaviour of wild juvenile lemon sharks, Negaprion brevirostris, by repeatedly subjecting sharks to open field tests. First, we investigated the presence of interindividual differences in movement behaviour in a novel open field. Second, we investigated the effect of trial repetition on movement behaviour to understand whether movement in a novel open field reflects a reaction to novelty, or general activity …