查看文章

PURPOSE:

The impact of an acute bout of exercise, especially high-intensity exercise, on the function of mitochondrial respiratory complexes is not well understood, with potential implications for both the healthy population and patients undergoing exercise based rehabilitation. Therefore, this study sought to comprehensively examine respiratory flux through the different complexes of the electron transport chain (ETC) in skeletal muscle mitochondria before and immediately after high-intensity aerobic exercise.

METHOD:

Muscle biopsies of the vastus lateralis were obtained at baseline and immediately following a 5 km time-trial performed on a cycle ergometer. Mitochondrial respiratory flux through the complexes of the ETC was measured in permeabilized skeletal muscle fibers by high-resolution respirometry.

RESULTS:

Complex I+ II state 3 respiration (state 3 CI+ CII), a measure of oxidative phosphorylation …