作者
Long Zhao, Xinyi Zhao, Tian Tian, Quanlong Lu, Nirma Skrbo-Larssen, Di Wu, Zheng Kuang, Xiaofeng Zheng, Yanchao Han, Shuyan Yang, Chuanmao Zhang, Anming Meng
发表日期
2008/11/1
期刊
Cardiovascular research
卷号
80
期号
2
页码范围
200-208
出版商
Oxford University Press
简介
Aims
Tropomyosin (Tpm) proteins, encoded by four Tpm genes (Tpm1–4), are associated with the stabilization of the F-actin filaments and play important roles in modulating muscle contraction. So far, little is known about Tpm4 function in embryonic heart development and its involvement in the cardiovascular diseases. In this study, we investigated functions of different isoforms of tpm4 in embryonic heartbeat in zebrafish.
Methods and results
The transgenic zebrafish line, T2EGEZ8, was generated by insertion of a Tol2 transposon gene trap vector, and homozygous mutants (T2EGEZ8m/m) of this line showed failure of embryonic heartbeat without other detectable phenotypes. Observation by transmission electron microscopy revealed that the ventricular myocytes of mutant fish contained fewer, disorganized myofibrillar filaments. The transposon genome in T2EGEZ8 fish was …
学术搜索中的文章
L Zhao, X Zhao, T Tian, Q Lu, N Skrbo-Larssen, D Wu… - Cardiovascular research, 2008