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A global analysis of the diet of chondrichthyes (23 species) was conducted during one bottom-trawl research cruise in the region where the highest catches of skates occurred off southwestern Atlantic. The diet was quantified by calculating the percentage frequency of occurrence of each prey. The influence of variables total length, sex, maturity stage, predator species, morphtype (sharks, skates, batoids), and depth on the diet was evaluated by fitting generalized linear models. The diet composition of chondrichthyes consisted mainly of crustaceans, polychaetes, and fish. The study region constituted a feeding ground for the majority of chondrichthyans species analyzed. The diet of chondrichthyes exhibited inter-specific differences sorted into three major trophic guilds and global ontogenetic shift. The consumption of crustaceans and polychaetes decreases with the total length of the chondrichthyes …