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Over the years, improved understanding of the nature of bone-implant interface is among the important contributors to the success of osseointegration in modern dental implantology. The focus has since shifted to the assessment of the soft tissue-implant interface to better understand the mechanism of biological seal in the transmucosal region. The importance of peri-implant mucosal region lies in the need to establish a tight seal that isolates implant and the bone from the oral environment via epithelial and connective tissue attachment, thus preventing ingrowth of bacterial plaque. Many factors may influence the soft tissue attachment at this peri-implant interface. In this chapter, the dimension of peri-implant tissues and the factors affecting the biological seal, namely surface topography and physicochemical properties, are discussed. The review also looks into the impact of the type of materials and surface modifications of dental implant, all of which may influence the formation of biological seal of soft tissue around the dental implant.