作者
K Maaser, M Höpfner, H Kap, AP Sutter, B Barthel, B Von Lampe, M Zeitz, H Scherübl
发表日期
2002/2
期刊
British journal of cancer
卷号
86
期号
4
页码范围
636-644
出版商
Nature Publishing Group
简介
Extracellular ATP is known to inhibit growth of various tumours by activating specific purinergic receptors (P2-receptors). Since the therapy of advanced oesophageal cancer is unsatisfying, new therapeutic approaches are mandatory. Here, we investigated the functional expression and potential antiproliferative effects of P2-purinergic receptors in human oesophageal cancer cells. Prolonged incubation of primary cell cultures of human oesophageal cancers as well as of the squamous oesophageal cancer cell line Kyse-140 with ATP or its stable analogue ATPγS dose-dependently inhibited cell proliferation. This was due to both an induction of apoptosis and cell cycle arrest. The expression of P2-receptors was examined by RT-PCR, immunocytochemistry, and [Ca 2+] i-imaging. Application of various extracellular nucleotides dose-dependently increased [Ca 2+] i. The rank order of potency was ATP= UTP> ATPγS …
引用总数
学术搜索中的文章
K Maaser, M Höpfner, H Kap, AP Sutter, B Barthel… - British journal of cancer, 2002