作者
Xuegang Yuan, Li Sun, Richard Jeske, Dingani Nkosi, Sara B York, Yuan Liu, Samuel C Grant, David G Meckes Jr, Yan Li
发表日期
2022/6
期刊
Journal of extracellular vesicles
卷号
11
期号
6
页码范围
e12235
简介
Human mesenchymal stem cell (hMSC) derived extracellular vesicles (EVs) have shown therapeutic potential in recent studies. However, the corresponding therapeutic components are largely unknown, and scale‐up production of hMSC EVs is a major challenge for translational applications. In the current study, hMSCs were grown as 3D aggregates under wave motion to promote EV secretion. Results demonstrate that 3D hMSC aggregates promote activation of the endosomal sorting complexes required for transport (ESCRT)‐dependent and ‐independent pathways. mRNA sequencing revealed global transcriptome alterations for 3D hMSC aggregates. Compared to 2D‐hMSC‐EVs, the quantity of 3D‐hMSC‐EVs was enhanced significantly (by 2‐fold), with smaller sizes, higher miR‐21 and miR‐22 expression, and an altered protein cargo (e.g., upregulation of cytokines and anti‐inflammatory factors …
学术搜索中的文章
X Yuan, L Sun, R Jeske, D Nkosi, SB York, Y Liu… - Journal of extracellular vesicles, 2022