作者
Peter M Kasson, Joshua D Rabinowitz, Lutz Schmitt, Mark M Davis, Harden M McConnell
发表日期
2000/2/8
期刊
Biochemistry
卷号
39
期号
5
页码范围
1048-1058
出版商
American Chemical Society
简介
Class II MHC glycoproteins bind short (7−25 amino acid) peptides in an extended type II polyproline-like conformation and present them for immune recognition. Because empty MHC is unstable, measurement of the rate of the second-order reaction between peptide and MHC is challenging. In this report, we use dissociation of a pre-bound peptide to generate the active, peptide-receptive form of the empty class II MHC molecule I−Ek. This allows us to measure directly the rate of reaction between active, empty I−Ek and a set of peptides that vary in structure. We find that all peptides studied, despite having highly variable dissociation rates, bind with similar association rate constants. Thus, the rate-limiting step in peptide binding is minimally sensitive to peptide side-chain structure. An interesting complication to this simple model is that a single peptide can sometimes bind to I−Ek in two kinetically distinguishable …
引用总数
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PM Kasson, JD Rabinowitz, L Schmitt, MM Davis… - Biochemistry, 2000