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Root knot nematode (Meloidogyne incognita) and soft rot bacterium Pectobacterium carotovorum subsp. carotovorum are major pathogens of carrot (Daucus carota) throughout the world, reducing both quality and quantity of marketable yield. Bacillus subtilis IIHR BS-2, a potential biocontrol agent, was evaluated against the nematode-bacterium disease complex in carrot under in vitro and field conditions. Under in vitro, culture filtrates of B. subtilis not only exhibited strong nematicidal activity by inhibiting the hatching of M. incognita eggs (94.65%) and causing juvenile mortality (91.26%), but also inhibited the growth of P. carotovorum (60.6%). Liquid formulation of B. subtilis IIHR BS-2 (CFU −1 × 10⁸ per ml) was tested under field conditions as seed treatment (10 ml kg⁻¹ seeds) and soil application (after enrichment in vermicompost), individually and in combination, and compared with application of chemicals …