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Enzyme cascade reactions for the synthesis of long chain aliphatic amines such as (Z)‐12‐aminooctadec‐9‐enoic acid, 10‐ or 12‐aminooctadecanoic acid, and 10‐amino‐12‐hydroxyoctadecanoic acid from renewable fatty acids were investigated. (Z)‐12‐aminooctadec‐9‐enoic acid was produced from ricinoleic acid ((Z)‐12‐hydroxyoctadec‐9‐enoic acid) via (Z)‐12‐ketooctadec‐9‐enoic acid with a conversion of 71% by a two‐step in vivo biotransformation involving a long chain secondary alcohol dehydrogenase (SADH) from Micrococcus luteus and a variant of the amine transaminase (ATA) from Vibrio fluvialis. 10‐Aminooctadecanoic acid was prepared from oleic acid ((Z)‐octadec‐9‐enoic acid) via 10‐hydroxyoctadecanoic acid and 10‐ketooctadecanoic acid by an in vivo three‐step biocatalysis reaction involving not only the SADH and ATA variants, but also a fatty acid double bond hydratase (OhyA) from …