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Materials and Methods: Angus-Holstein heifers (n= 41) underwent estrus-synchronization and were divided into groups:(1) artificial insemination to produce in vivo-derived conceptuses,(2) embryo transfer to produce in vitro-produced conceptuses, or (3) remained cyclic to produce synchronized endometrium. On Day 16, uteri were harvested and endometrial-explants from cyclic animals and conceptuses from pregnant animals were used to develop treatments in 1mL RPMI medium:(1) endometrium alone (Control; 8 mm; n= 9),(2) endometrium cultured with an in vivo-derived conceptus (5× 2 mm; n= 10),(3) endometrium cultured with an in vitro-produced conceptus (5× 2 mm; n= 10),(4) in vivo-derived conceptus alone (5× 2 mm; n= 7) and (5) in vitro-produced conceptus alone (5× 2 mm; n= 7). Medium was also cultured without conceptus or endometrial tissue (n= 7). After 12 h, media was collected for metabolomic …