作者
Minbiao Ji, Spencer Lewis, Sandra Camelo-Piragua, Shakti H Ramkissoon, Matija Snuderl, Sriram Venneti, Amanda Fisher-Hubbard, Mia Garrard, Dan Fu, Anthony C Wang, Jason A Heth, Cormac O Maher, Nader Sanai, Timothy D Johnson, Christian W Freudiger, Oren Sagher, Xiaoliang Sunney Xie, Daniel A Orringer
发表日期
2015/10/14
期刊
Science translational medicine
卷号
7
期号
309
页码范围
309ra163-309ra163
出版商
American Association for the Advancement of Science
简介
Differentiating tumor from normal brain is a major barrier to achieving optimal outcome in brain tumor surgery. New imaging techniques for visualizing tumor margins during surgery are needed to improve surgical results. We recently demonstrated the ability of stimulated Raman scattering (SRS) microscopy, a nondestructive, label-free optical method, to reveal glioma infiltration in animal models. We show that SRS reveals human brain tumor infiltration in fresh, unprocessed surgical specimens from 22 neurosurgical patients. SRS detects tumor infiltration in near-perfect agreement with standard hematoxylin and eosin light microscopy (κ = 0.86). The unique chemical contrast specific to SRS microscopy enables tumor detection by revealing quantifiable alterations in tissue cellularity, axonal density, and protein/lipid ratio in tumor-infiltrated tissues. To ensure that SRS microscopic data can be easily used in brain …
学术搜索中的文章
Detection of human brain tumor infiltration with quantitative stimulated Raman scattering microscopy
M Ji, S Lewis, S Camelo-Piragua, SH Ramkissoon… - Science translational medicine, 2015