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Objective: VEGF plays an important role in tumor growth, development and progression by promoting angiogenesis. However its role in oral carcinogenesis is not established. In the present study we investigated serum and tissue expression of VEGF in oral cancer patients and its correlation with clinicopathological parameters; as well as with tumor cell proliferation using OSCC cell lines.

Materials and Methods: Cell and tissue expression of VEGF was determined by qRT-PCR, western blot and immunofluorescence assay while serum level of VEGF was determined by ELISA. Tumor cell proliferation was assessed by MTT assay.

Results: VEGF mRNA in tissue was significantly upregulated in tumor area compared to normal area of the same specimen as observed by qRT-PCR (R=7.28±1.40; p= 0.029) and western blot assay (p=0.0345). Significantly higher expression of VEGF was observed in sera of oral cancer …