作者
Glenn Rademakers, Maartje Massen, Alexander Koch, Muriel X Draht, Nikkie Buekers, Kim AD Wouters, Nathalie Vaes, Tim De Meyer, Beatriz Carvalho, Gerrit A Meijer, James G Herman, Kim M Smits, Manon van Engeland, Veerle Melotte
发表日期
2021/12
期刊
Clinical Epigenetics
卷号
13
期号
1
页码范围
1-12
出版商
BioMed Central
简介
Purpose
Colonoscopy and the fecal immunochemical test (FIT) are currently the most widely used screening modalities for colorectal cancer (CRC), however, both with their own limitations. Here we aim to identify and validate stool-based DNA methylation markers for the early detection of CRC and investigate the biological pathways prone to DNA methylation.
Methods
DNA methylation marker discovery was performed using The Cancer Genome Atlas (TCGA) colon adenocarcinoma data set consisting of normal and primary colon adenocarcinoma tissue. The performance of the five best candidate markers and a previously identified marker, NDRG4, was evaluated on tissues and whole stool samples of healthy subjects and CRC patients using quantitative MSP assays. The results were compared and combined with FIT data. Finally, pathway and gene …
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G Rademakers, M Massen, A Koch, MX Draht… - Clinical epigenetics, 2021