查看文章

Glycogen synthase kinase 3 beta (GSK-3β) is constantly active in cells and its activity increases after serum deprivation, indicating that GSK-3β might play a major role in cell survival under serum starvation. In this study, we attempted to determine how GSK-3β promotes cell survival after serum depletion. Under full culture conditions (10% FBS), GSK-3β inhibition with chemical inhibitors or siRNAs failed to induce cell death in human prostate cancer cells. By contrast, under conditions of serum starvation, a profound necrotic cell death was observed as evidenced by cellular morphologic features and biochemical markers. Further analysis revealed that GSK-3β-inhibition-induced cell death was in parallel with an extensive autophagic response. Interestingly, blocking the autophagic response switched GSK-3β-inhibition-induced necrosis to apoptotic cell death. Finally, GSK-3β inhibition resulted in a remarkable …