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Biological invasions exert multiple pervasive effects on ecosystems, potentially disrupting species interactions and global ecological processes. Our ability to successfully predict and manage the ecosystem-level impacts of biological invasions is strongly dependent on our capacity to empirically characterize complex biological interactions and their spatiotemporal dynamics. In this chapter, we argue that the comprehensive integration of multiple complementary tools within the explicit context of ecological networks is essential for providing mechanistic insight into invasion processes and their impact across organizational levels. We provide an overview of traditional (stable isotopes, populations genetics) and emerging (metabarcoding, citizen science) techniques and methods, and their practical implementation in the context of biological invasions. We also present several currently available models and machine …