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Biohydrogen production from microalgae still remains to be discussed and examined more specifically, given that it is one of the most important energy carriers possessing environmental-friendly and sustainable characteristics. Although microalgae species capable of biohydrogen production do exist, Chlamydomonas reinhardtii is considered to be one of the most promising eukaryotic H₂ producers, and can serve as a model organism for such studies. Unfortunately, even if the metabolic basis and environmental conditions for this process are well defined, the sustainability of biohydrogen production is not straightforward. At this point, genetic engineering tools must be efficacious in order to enable mutant strains to reach desired amounts of biohydrogen. In this study, different light intensities, illumination patterns and Chlamydomonas strains such as CC124 and D1 protein mutant strains (D240, D239-40, D240-41 …