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The growth rate of H. pluvialis is controlled or regulated by the physical and chemical parameters. The aim of this study was to investigate and compare the effect of various culture media and light intensities on the growth of H. pluvialis in batch culture. The experiments were achieved by five different culture media and three different light intensities. The maximum cell concentration of 9.50 x 105 cells ml-1, which corresponds to the growth rate of 0.195 d-1, was obtained in RM culture medium at the light intensity of 40 µmol photons m-2 s-1. The cell concentration decreased by only 7% in Basal culture medium compared to RM culture medium under the same light intensity.