作者
Seyed Jalal Hashemi, Ahmad Farajzadeh Sheikh, Hamed Goodarzi, Mohammad Jaafar Yadyad, Seyed Saeid Seyedian, Sajad Aslani, Mohammad-Ali Assarzadegan
发表日期
2019/3/4
期刊
Infection and Drug Resistance
页码范围
535-543
出版商
Taylor & Francis
简介
Background
The aim of this study was to evaluate the antimicrobial resistance and genetic basis for metronidazole (Mtz) and clarithromycin (Cla) resistance in strains of Helicobacter pylori, isolated from patients with gastroduodenal disorders.
Patients and methods
A total of 157 H. pylori isolates (from 22 gastric cancer, 38 peptic ulcer disease, and 97 non-ulcer dyspepsia patients) were analyzed for drug susceptibility to Mtz and Cla, by gradient diffusion test (E-test, MAST). The PCR and sequence analysis of the rdxA and frxA for Mtz-resistant strains and the 23S rRNA for Cla-resistant strains were used to determine the genetic basis of drug resistance in H. pylori strains. Increased expression of TolC homologous genes (hefA) that upregulates efflux pump activity was determined in multidrug-resistant (MDR) strain of H. pylori by real-time PCR technique.
Results
Among 157 H. pylori isolates, 32 (20.4%) strains were …
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SJ Hashemi, AF Sheikh, H Goodarzi, MJ Yadyad… - Infection and Drug Resistance, 2019