作者
Yanyong Kang, X Edward Zhou, Xiang Gao, Yuanzheng He, Wei Liu, Andrii Ishchenko, Anton Barty, Thomas A White, Oleksandr Yefanov, Gye Won Han, Qingping Xu, Parker W De Waal, Jiyuan Ke, MH Eileen Tan, Chenghai Zhang, Arne Moeller, Graham M West, Bruce D Pascal, Ned Van Eps, Lydia N Caro, Sergey A Vishnivetskiy, Regina J Lee, Kelly M Suino-Powell, Xin Gu, Kuntal Pal, Jinming Ma, Xiaoyong Zhi, Sébastien Boutet, Garth J Williams, Marc Messerschmidt, Cornelius Gati, Nadia A Zatsepin, Dingjie Wang, Daniel James, Shibom Basu, Shatabdi Roy-Chowdhury, Chelsie E Conrad, Jesse Coe, Haiguang Liu, Stella Lisova, Christopher Kupitz, Ingo Grotjohann, Raimund Fromme, Yi Jiang, Minjia Tan, Huaiyu Yang, Jun Li, Meitian Wang, Zhong Zheng, Dianfan Li, Nicole Howe, Yingming Zhao, Jörg Standfuss, Kay Diederichs, Yuhui Dong, Clinton S Potter, Bridget Carragher, Martin Caffrey, Hualiang Jiang, Henry N Chapman, John CH Spence, Petra Fromme, Uwe Weierstall, Oliver P Ernst, Vsevolod Katritch, Vsevolod V Gurevich, Patrick R Griffin, Wayne L Hubbell, Raymond C Stevens, Vadim Cherezov, Karsten Melcher, H Eric Xu
发表日期
2015/7/30
期刊
Nature
卷号
523
期号
7562
页码范围
561-567
出版商
Nature Publishing Group UK
简介
G-protein-coupled receptors (GPCRs) signal primarily through G proteins or arrestins. Arrestin binding to GPCRs blocks G protein interaction and redirects signalling to numerous G-protein-independent pathways. Here we report the crystal structure of a constitutively active form of human rhodopsin bound to a pre-activated form of the mouse visual arrestin, determined by serial femtosecond X-ray laser crystallography. Together with extensive biochemical and mutagenesis data, the structure reveals an overall architecture of the rhodopsin–arrestin assembly in which rhodopsin uses distinct structural elements, including transmembrane helix 7 and helix 8, to recruit arrestin. Correspondingly, arrestin adopts the pre-activated conformation, with a ∼20° rotation between the amino and carboxy domains, which opens up a cleft in arrestin to accommodate a short helix formed by the second intracellular loop of rhodopsin …
学术搜索中的文章
Y Kang, XE Zhou, X Gao, Y He, W Liu, A Ishchenko… - Nature, 2015