作者
Minjung Park, Ju-Hoon Lee, Hakdong Shin, Minsik Kim, Jeongjoon Choi, Dong-Hyun Kang, Sunggi Heu, Sangryeol Ryu
发表日期
2012/1/1
期刊
Applied and environmental microbiology
卷号
78
期号
1
页码范围
58-69
出版商
American Society for Microbiology
简介
Salmonella enterica and Escherichia coli O157:H7 are major food-borne pathogens causing serious illness. Phage SFP10, which revealed effective infection of both S. enterica and E. coli O157:H7, was isolated and characterized. SFP10 contains a 158-kb double-stranded DNA genome belonging to the Vi01 phage-like family Myoviridae. In vitro adsorption assays showed that the adsorption constant rates to both Salmonella enterica serovar Typhimurium and E. coli O157:H7 were 2.50 × 10−8 ml/min and 1.91 × 10−8 ml/min, respectively. One-step growth analysis revealed that SFP10 has a shorter latent period (25 min) and a larger burst size (>200 PFU) than ordinary Myoviridae phages, suggesting effective host infection and lytic activity. However, differential development of resistance to SFP10 in S. Typhimurium and E. coli O157:H7 was observed; bacteriophage-insensitive mutant (BIM) frequencies of 1.19 × …
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M Park, JH Lee, H Shin, M Kim, J Choi, DH Kang… - Applied and environmental microbiology, 2012