作者
Peter Møller, Amaya Azqueta, Elisa Boutet-Robinet, Gudrun Koppen, Stefano Bonassi, Mirta Milić, Goran Gajski, Solange Costa, João Paulo Teixeira, Cristiana Costa Pereira, Maria Dusinska, Roger Godschalk, Gunnar Brunborg, Kristine B Gutzkow, Lisa Giovannelli, Marcus S Cooke, Elke Richling, Blanca Laffon, Vanessa Valdiglesias, Nursen Basaran, Cristian Del Bo’, Bojana Zegura, Matjaz Novak, Helga Stopper, Pavel Vodicka, Sona Vodenkova, Vanessa Moraes de Andrade, Monika Sramkova, Alena Gabelova, Andrew Collins, Sabine AS Langie
发表日期
2020/12
来源
Nature protocols
卷号
15
期号
12
页码范围
3817-3826
出版商
Nature Publishing Group UK
简介
The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between ‘desirable’ and ‘essential’ information: ‘essential’ information refers to the precise details that are necessary to assess the quality of the experimental work, whereas ‘desirable’ information relates to technical issues that might be encountered when repeating the experiments …
学术搜索中的文章
P Møller, A Azqueta, E Boutet-Robinet, G Koppen… - Nature protocols, 2020