作者
Cátia V Vaz, Ricardo Marques, Marco G Alves, Pedro F Oliveira, José E Cavaco, Cláudio J Maia, Sílvia Socorro
发表日期
2016/1/1
期刊
Journal of cancer research and clinical oncology
卷号
142
期号
1
页码范围
5-16
出版商
Springer Berlin Heidelberg
简介
Purpose
The present study aims to investigate the role of androgens in controlling the glycolytic metabolism and lactate efflux in prostate cancer (PCa) cells.
Methods
Androgen-responsive LNCaP cells were treated with 5α-dihydrotestosterone (DHT, 10 nM) for 12–48 h, and their glycolytic metabolism, lactate production and viability were analyzed. Intracellular and extracellular levels of glucose and lactate were determined spectrophotometrically, and the expression of glucose transporters (GLUT1/GLUT3), phosphofructokinase 1, lactate dehydrogenase (LDH) and monocarboxylate transporter (MCT4) was analyzed by real-time PCR and Western blot. The enzymatic activity of LDH was determined by means of a colorimetric assay. Experiments were reproduced in androgen-non-responsive DU145 and PC3 cells.
Results …
引用总数
201620172018201920202021202220232024561671512765
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