作者
PF Oliveira, MG Alves, L Rato, S Laurentino, J Silva, R Sa, A Barros, M Sousa, RA Carvalho, JE Cavaco, S Socorro
发表日期
2012/2/1
期刊
Biochimica et Biophysica Acta (BBA)-General Subjects
卷号
1820
期号
2
页码范围
84-89
出版商
Elsevier
简介
BACKGROUND
Sertoli cells metabolize glucose producing lactate for developing germ cells. As insulin regulates glucose uptake and its disturbance/insensitivity is associated with diabetes mellitus, we aimed to determine the effect of insulin deprivation in human Sertoli cell (hSC) metabolism and metabolism-associated gene expression.
METHODS
hSC-enriched primary cultures were maintained in the absence/presence of insulin and metabolite variations were determined by 1H-NMR. mRNA expression levels of glucose transporters (GLUT1, GLUT3), lactate dehydrogenase (LDHA) and monocarboxylate transporter (MCT4) were determined by RT-PCR.
RESULTS
Insulin deprivation resulted in decreased lactate production and in decrease of glucose consumption that was completely reverted after 6h. Cells of both groups consumed similar amounts of glucose. In insulin-deprived cells, transcript levels of genes …
引用总数
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