查看文章

The effect of mechanical wounding on alkaloid metabolism was analyzed in Catharanthus roseus seedlings. Wounding induced an increase in ajmalicine accumulation, whereas catharanthine remained unaffected. A positive dual effect on vindoline was noticed. Short and mid-term effects were detected between 12 and 24 h after mechanical damage was inflicted, and apparently resulted from the accelerated transformation of the tabersonine intermediaries to vindoline. Long-term effects involved a generalized increase in carbon flux towards alkaloid synthesis. Exposure to ethylene (1 ppm) produced similar results to those observed in wounded seedlings, suggesting that it might be mediating the wound-induced increase in alkaloid synthesis. No synergistic or additive effects were observed when wounded seedlings were exposed to ethylene or jasmonate.