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The objective of this study was to reestablish an extinct strain of sheep that exhibits spontaneous X-linked factor VIII deficiency closely mimicking human hemophilia A. Twenty female carriers of the trait, produced in a previous study (Bormann et al. 2006 Reprod. Fertil. Dev. 18, 201–202), were backcrossed using 3 straws of semen from their affected sire using either IVF or multiple ovulation embryo transfer (MOET). Eleven oocyte donors were synchronized with CIDRs (15 days) and superovulated with a declining dose of FSH (204 mg) twice daily for 3.5 days. Nine MOET donors were synchronized using CIDRs (14 days), superovulated with a declining dose of FSH (184 mg) BID for 3 days with pregnant mare serum gonadotropin (PMSG; 200 IU) given with the final dose of FSH, and given 1000 IU of hCG 12 h post-CIDR removal. Recipient ewes were synchronized using sponges (Ovakron, HeriotAgvet, Rowville …