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This work characterizes the impacts of the realistic roughness due to deposition of foreign materials on the turbulent flows at surface transition from elevated rough-wall to smooth-wall. High resolution PIV measurements were performed in the streamwise-wall-normal (x–y) planes at two different spanwise positions in both smooth and rough backward-facing step flows. The experiment conditions were set at a Reynolds number of 3450 based on the free stream velocity U_∞ and the mean step height h, expansion ratio of 1.01, and the ratio of incoming boundary layer thickness to the step height, δ/h, of 8. The mean flow structures are observed to be modified by the roughness and they illustrate three-dimensional features in rough backward-facing step flows. The mean reattachment length X_r is significantly reduced by the roughness at one PIV measurement position while is slightly increased by the different roughness …