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Asthma is a chronic inflammatory disease with structural changes in the airway wall, including goblet cell metaplasia, loss of ciliated epithelial cells, basement membrane thickening and airway smooth muscle hypertrophy and hyperplasia. To characterize the cellular composition of airway wall in asthma, and identify the associated molecular changes in each cell type individually, we performed single-cell RNA sequencing of airway wall biopsies from asthma patients and matched healthy controls. Airway wall biopsies from subsegmental carinae (3^rd to 6^th generation) were obtained by bronchoscopy from patients with asthma (confirmed by provocation test) and matched healthy controls. Suspensions of live biopsy cells were partitioned using a 10XGenomics Chromium controller. We generated and sequenced single-cell libraries for over 15000 cells. Cluster analysis revealed presence of at least 20 discrete cell …