查看文章

Purpose:: Cell penetrating dendrimeric polyguanidilyated translocators (DPTs), a new class of polymers, potentially enhance topical ocular delivery across epithelial barriers. Ophthalmic drop dosage forms require high drug solubility. The study objective was to determine whether a g6 DPT dendrimer is suitable for enhancing the solubility of gatifloxacin (zwitterion), ketorolac tromethamine (KT, negatively charged), and tropicamide (positively charged), three topical ophthalmic drugs.

Methods:: Equilibrium drug solubility studies in the presence of 1 and 10 mg/ml dendrimer were carried out in isotonic PBS (pH 5.65, 6.30, and 7.37) using HPLC analysis. Drug and dendrimer interactions were characterized using isothermal titration calorimetry (ITC) and Fourier-transformed infrared spectroscopy (FTIR). Molecular size of the dendrimer was ensured using membrane filtration.

Results:: The dendrimer was highly soluble (> …