作者
Nehal Gosalia, Daniel Neems, Jenny L Kerschner, Steven T Kosak, Ann Harris
发表日期
2014/7/31
期刊
Nucleic acids research
卷号
42
期号
15
页码范围
9612-9622
出版商
Oxford University Press
简介
Higher order chromatin structures across the genome are maintained in part by the architectural proteins CCCTC binding factor (CTCF) and the cohesin complex, which co-localize at many sites across the genome. Here, we examine the role of these proteins in mediating chromatin structure at the cystic fibrosis transmembrane conductance regulator (CFTR) gene. CFTR encompasses nearly 200 kb flanked by CTCF-binding enhancer-blocking insulator elements and is regulated by cell-type-specific intronic enhancers, which loop to the promoter in the active locus. SiRNA-mediated depletion of CTCF or the cohesin component, RAD21, showed that these two factors have distinct roles in regulating the higher order organization of CFTR. CTCF mediates the interactions between CTCF/cohesin binding sites, some of which have enhancer-blocking insulator activity. Cohesin shares this tethering role, but in …
学术搜索中的文章
N Gosalia, D Neems, JL Kerschner, ST Kosak, A Harris - Nucleic acids research, 2014