作者
Rui Yang, Jenny L Kerschner, Nehal Gosalia, Daniel Neems, Lidija K Gorsic, Alexias Safi, Gregory E Crawford, Steven T Kosak, Shih-Hsing Leir, Ann Harris
发表日期
2016/4/20
期刊
Nucleic acids research
卷号
44
期号
7
页码范围
3082-3094
出版商
Oxford University Press
简介
Higher order chromatin structure establishes domains that organize the genome and coordinate gene expression. However, the molecular mechanisms controlling transcription of individual loci within a topological domain (TAD) are not fully understood. The cystic fibrosis transmembrane conductance regulator (CFTR) gene provides a paradigm for investigating these mechanisms. CFTR occupies a TAD bordered by CTCF/cohesin binding sites within which are cell-type-selective cis-regulatory elements for the locus. We showed previously that intronic and extragenic enhancers, when occupied by specific transcription factors, are recruited to the CFTR promoter by a looping mechanism to drive gene expression. Here we use a combination of CRISPR/Cas9 editing of cis-regulatory elements and siRNA-mediated depletion of architectural proteins to determine the relative contribution of structural elements and …
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R Yang, JL Kerschner, N Gosalia, D Neems, LK Gorsic… - Nucleic acids research, 2016