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The recent advancements in biotechnology and biomarkers discovery are now refining many biological systems in disease and diagnostic. The growing demand of application of biosensors in the field of medical diagnostic is because of its high sensitivity and multifunctional performances by a single chip. Currently, biosensor is widely used in health care, particularly diagnosis of infectious diseases as the number of predicted deaths remains high, with the pandemics and epidemics hazards and antibiotics resistance to various pathogens. The current scenario of biosensor technologies has potential to convey bed-side diagnostics, which matches traditional standards with respect to cost, accuracy, and time. Nevertheless, constant refinement in the technology is needed in order to provide lab-on-chip rapid, affordable, and accurate diagnostic. In this chapter, the importance of biosensor to diagnose several infectious …