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The Multi-Dimensional Voice Program (MDVP) and Praat are computer programs commonly used for acoustic analysis of voice in clinical and research settings. Both softwares extract a set of acoustic parameters, many of which are defined similarly. The purpose of this study was to compare results obtained by both programs, and examine whether they can clinically distinguish among pathological groups differently. Fifty-eight women participated in the study. Of these women, 28 were diagnosed with functional dysphonia and 30 were diagnosed with benign mass-lesions (10 nodules, ten polyps and 10 cysts). Six productions of the vowels /a/ and /i/, were analyzed using MDVP and Praat. Results show similar mean fundamental-frequency (mF0) values for both programs (P>0.05). However, values of jitter, shimmer, noise-to-harmonic ratio (NHR) and degree of unvoiced (DUV) segment were significantly lower using …