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Synthesis of nanosized particles with antimicrobial property is of significance in therapeutic applications of nanotechnology. The present study reports an environmental friendly and rapid method for biosynthesis of silver nanoparticles and their antibacterial activity. This green synthetic method used various fruit juices like sweet lime, lime and orange as a reducing and capping agent for silver nitrate. Different thermal reduction methods-microwave oven and hot plate were used for synthesizing silver nanoparticles. The silver ions were reduced into silver nanoparticles within few minutes of reaction. Silver nanoparticles so prepared were characterized using UV–visible spectrophotometer and scanning electron microscope. An effort was also been made to predict the size of the silver nanoparticles using UV-visible spectra by Mie Scattering protocol. Scanning electron micrograph (SEM) revealed useful information about the morphology of silver nanoparticles. The synthesized silver nanoparticles showed antibacterial property against pathogenic bacteria Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae and Staphylococcus aureus. Agar well diffusion method was adopted to assay the nanoparticles for bactericidal activity against test organisms on nutrient agar plates. Silver nanoparticles biosynthesized from orange fruit juice were more effective as antibacterial agent. The maximum zones of inhibition of 8.0 mm, 6.0 mm, 8.0 mm and 5.0 mm were observed against Escherichia coli, Salmonella typhi, Klebsiella pneumoniae and Staphylococcus aureus respectively. The study shows that gram negative bacteria are more susceptible to …