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This study demonstrated a solvothermal method of growth of three different morphologies of zinc oxide nanoparticles (ZnO NPs): i) flower-like nanorod and nanoflakes, ii) assembled hierarchical structure, and iii) nano granule. Oleic acid (C₁₈H₃₄O₂), gluconic acid (C₆H₁₂O₇) and tween 80 (C₆₄H₁₂₄O₂₆) were used as surfactant/capping/reducing agent for the formation of different morphologies of nanoparticles. The as-synthesized ZnO NPs were characterized by different physicochemical techniques such as UV–vis (UV–vis) spectroscopy, X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray (EDX) analysis and dynamic light scattering (DLS) studies. Further, the antioxidant and antimicrobial activity of these nanostructures was evaluated. The antioxidant activity of these nanostructures was assessed via 2,2-diphenyl …