作者
Meredith M Ogle, Ashleigh D Smith McWilliams, Matthew J Ware, Steven A Curley, Stuart J Corr, Angel A Martí
发表日期
2019/8/20
期刊
The Journal of Physical Chemistry B
卷号
123
期号
34
页码范围
7282-7289
出版商
American Chemical Society
简介
Boron dipyrromethene (BODIPY) molecular rotors have shown sensitivity toward viscosity, polarity, and temperature. Here, we report a 1,3,5,7-tetramethyl-8-phenyl-BODIPY modified with a polyethylene glycol (PEG) chain, for temperature sensing and live cell imaging. This new PEG-BODIPY dye presents an increase in nonradiative decay as temperature increases, which directly influences its lifetime. This change in lifetime is dependent on changes in both temperature and viscosity at low viscosity values, but is only dependent on temperature at high viscosity values. The dependence of fluorescence lifetime with temperature allows for temperature monitoring in vitro and in cells, with sub degree resolution. When in contact with cells, the PEG-BODIPY spontaneously penetrates and stains the cell but not the nucleus. Furthermore, no significant cell toxicity was found even at 100 μM concentration. Using …
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MM Ogle, AD Smith McWilliams, MJ Ware, SA Curley… - The Journal of Physical Chemistry B, 2019